Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

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Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma 1, Kpn 1, HindIII, Xba 1, Bgl I + II, and EcoRI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with ...

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Ribosomal RNA Genes of Saccharomyces cerevisiae I. PHYSICAL MAP OF THE REPEATING UNIT AND LOCATION OF THE REGIONS

The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, BgZ I + II, and EcoRI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with ...

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Secondary structure of 5 S ribosomal RNA.

A secondary structure model for 16S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations. The four major structural domains created by the base-pairing scheme correspond closely to RNA fragments isolated after nuclease digestion in the presence o...

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Magnification of genes coding for ribosomal RNA in Saccharomyces cerevisiae.

When a strain of Saccharomyces cerevisiae monosomic for chromosome I and initially deficient for 25% of the genes coding for ribosomal RNA is repeatedly subcultured, the number of these genes increases to and remains stable at the number in the wild type. This strain shows 2:2; viable: inviable first division segregation and hemizygosity for the ade1 gene (a chromosome I marker), evidence that ...

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The Phylogeny of Sp Based on the Partialecies of the Genus jlssatehenkia KuDRiAvzEv ( Saccharomycetaceae ) S qu nces of 18 S and 26 S Ribosomal RNAs

The ten strains of lssatchenkia species were examined for their partial base sequences of 18S and 26S rRNAs. In the 18S rRNA partial base seguences (positions 1451-1618, 168 bases), the strains of the species ef the genus fssatchenkia wu'ere found to be net uniform phylogenetically. The calculated base difibrences numbered 5-O. The straims of tssatchenkia species examined had 3-1 base differeRc...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1977

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)40947-1